What is in vivo?
生醫研究常常見到許多如in vivo, ex vivo, in vitro, in situ等拉丁文簡稱，但都沒有好好去分清楚他們的定義。上次在陽明神研所演講，還有一位老師說他們做histology也是in vivo，登時把我也搞糊塗了。
根據Wikipedia的定義，in vivo是指在完整未分離的活體中的實驗，所以histology這種在死動物上的，就不算。ex vivo則是將器官或細胞拿出體外活著養在人工環境中；in vitro則是在試管中（如試管嬰兒in vitro fertilization）。in situ則是沒有離體，可是又跟in vivo不同，可能是死的或perfusion狀態下。
所以，我的MRI實驗才是正港的in vivo啦！
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Research
2 comments:
Could you raise some example for this?
How about doing some protein western blot?Is it consider as ex vivo?
Western blot is always done outside the body, so it is ex vivo.
There are situation that is in between in vivo and ex vivo. For example, 14C-2DG autoradiography has to be imaged in tissue section. But the activity it recorded is actually in vivo in awake animal. So someone would say this technique in vivo.
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